The Endless Search – at 4ºC – for Novel RNA Processing Factors in Mammalian Cells
20 September 2018
Prof. Javier Martinez
Max F. Perutz Laboratories, Medical University of Vienna, Austria
- Research topic: Biochemistry, physiology and disease of the tRNA splicing pathway in mammalian cells
- Laboratory webpage: http://www.mfpl.ac.at/groups/mfpl-group/group-info/martinez.html
About the lecture
My talk will deal with the discovery and characterization of essential RNA processing enzymes in mammalian cells, particularly in the pre-tRNA splicing pathway. By studying those enzymes, we now understand (and enjoy) the metabolism of RNA molecules much better.
I will go back in time and describe the biology and biochemistry of:
- the 5’ RNA-kinase CLP1, an enzyme that associates to the tRNA splicing endonuclease (TSEN) complex and the mRNA 3’-end formation machinery;
- The tRNA ligase complex, a pentamer with HSPC117/RTCB as a catalytic subunit;
- Archease, an essential cofactor that turns the tRNA ligase into a multiple turnover enzyme.
In the second part of my talk, I will focus on a very exciting (and serendipitous) (and unpublished) finding in our Lab: We have identified the first 2’,3’-cyclic phosphatase activity in human cells. This is important because the biochemistry and function of RNAs with terminal 2’,3’-cyclic phosphates remain mysterious.
You will hear about the pathway to its discovery, its characterization and the future perspectives.
This lecture is sponsored by Twinning project BISON.