The Endless Search – at 4ºC – for Novel RNA Processing Factors in Mammalian Cells
20 September 2018
- University Campus Bohunice, pavilion A11, room 132
Prof. Javier Martinez
Max F. Perutz Laboratories, Medical University of Vienna, Austria
- Research topic: Biochemistry, physiology and disease of the tRNA splicing pathway in mammalian cells
- Laboratory webpage: http://www.mfpl.ac.at/groups/mfpl-group/group-info/martinez.html
About the lecture
My talk will deal with the discovery and characterization of essential RNA processing enzymes in mammalian cells, particularly in the pre-tRNA splicing pathway. By studying those enzymes, we now understand (and enjoy) the metabolism of RNA molecules much better.
I will go back in time and describe the biology and biochemistry of:
- the 5’ RNA-kinase CLP1, an enzyme that associates to the tRNA splicing endonuclease (TSEN) complex and the mRNA 3’-end formation machinery;
- The tRNA ligase complex, a pentamer with HSPC117/RTCB as a catalytic subunit;
- Archease, an essential cofactor that turns the tRNA ligase into a multiple turnover enzyme.
In the second part of my talk, I will focus on a very exciting (and serendipitous) (and unpublished) finding in our Lab: We have identified the first 2’,3’-cyclic phosphatase activity in human cells. This is important because the biochemistry and function of RNAs with terminal 2’,3’-cyclic phosphates remain mysterious.
You will hear about the pathway to its discovery, its characterization and the future perspectives.
Registration for lunch with the speaker /for PhD students/
Sponsored lunch with students takes place usually on Thursday before the lecture. The exact time and place will be specified after your registration.
This lecture is sponsored by Twinning project BISON.