New Frontiers in PKA Signaling

  • 16 November 2023
    4:00 PM
  • University Campus Bohunice (pavilion B11/ seminar room 132)

Lecture will be held in English


Hosted by

Jakub Harnoš

About the lecture


New Frontiers in PKA Signaling

Although the first crystal structure of the catalytic (C) subunit of cAMP-dependent protein kinase (PKA) was solved over 30 years ago, we are still learning new things about this ubiquitous kinase that regulates so much biology. In cells the C-subunit is stored as a holoenzyme complex with inhibitory regulatory (R) subunits that are activated by cAMP, and the quaternary structures created by each of the four functionally non-redundant R-subunits are remarkably different. These holoenzymes are then localized to specific sites in the cell, typically by A Kinase Anchoring Proteins (AKAPs) that create unique cAMP signaling islands. The isoform diversity of the C-subunit is also critical for mediating cell-specific signaling. While the well-studied Ca subunit serves as a prototype for the protein kinase superfamily, the Cb isoforms have been largely ignored although they account for nearly 50% of PKA signaling in brain. In parallel with our structural studies of PKA subunits and full-length holoenzymes, it is essential to demonstrate how PKA subunits are localized cells and tissues, and here we are using the retina as a “Window into the Brain”. Human mutations are also providing important clues that are allowing us to decipher how defects in PKA signaling can drive a variety of diseases. Our multi-scale approach that extends from solving high resolution structures to imaging in cells and tissues continues to lead to new discoveries that include non-canonical mechanisms for PKA signaling.


Registration for lunch with the speaker /for Ph.D. students/

The sponsored lunch usually takes place in the Campus River restaurant. Please meet the speaker and other students at 12:45 at the reception desk at the main entrance (building B22, see the map below).


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